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clontech yeast matchmaker lexa system  (Becton Dickinson)
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Becton Dickinson clontech yeast matchmaker lexa system
The two-hybrid vectors pGilda and pB42AD contained the <t>LexA</t> <t>DNA-binding</t> domain and the B42 activation domain, respectively. Yeast EGY48 cells were transformed with either one or two plasmids as follows. A, pGildaFKBP39 (1), pB42ADChd64 (2), pGildaFKBP39 + pB42ADChd64 (3), pGildaChd64 (4), pB42ADFKBP39 (5), and pGildaChd64 + pB42ADFKBP39 (6). B, pGildaEcR + pB42ADFKBP39 (1), pGildaEcR + pB42ADChd64 (2), pGildaUSP + pB42ADFKBP39 (3), pGildaUSP + pB42ADChd64 (4), pGildaMet + pB42ADFKBP39 (5), pGildaMet + pB42ADChd64 (6), pGildaFKBP39 + pB42ADEcR (7), pGildaChd64 + pB42ADEcR (8), pGildaFKBP39 + pB42ADUSP (9), pGildaChd64 + pB42ADUSP (10), PGildaFKBP39 + pB42ADMet (11), pGild-aChd64 + pB42ADMet (12), pGildaEcR (13), pGildaUSP (14), pGildaMet (15), pB42ADEcR (16), pB42ADUSP (17), and pB42ADMet (18). The expression of the two-hybrid target gene, β-galactosidase, was monitored by filter assays using X-gal as the enzyme substrate. Expression of β-galactosidase turned yeast colonies blue in the presence of X-gal, indicating physical interactions between the two proteins tested.
Clontech Yeast Matchmaker Lexa System, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The two-hybrid vectors pGilda and pB42AD contained the LexA DNA-binding domain and the B42 activation domain, respectively. Yeast EGY48 cells were transformed with either one or two plasmids as follows. A, pGildaFKBP39 (1), pB42ADChd64 (2), pGildaFKBP39 + pB42ADChd64 (3), pGildaChd64 (4), pB42ADFKBP39 (5), and pGildaChd64 + pB42ADFKBP39 (6). B, pGildaEcR + pB42ADFKBP39 (1), pGildaEcR + pB42ADChd64 (2), pGildaUSP + pB42ADFKBP39 (3), pGildaUSP + pB42ADChd64 (4), pGildaMet + pB42ADFKBP39 (5), pGildaMet + pB42ADChd64 (6), pGildaFKBP39 + pB42ADEcR (7), pGildaChd64 + pB42ADEcR (8), pGildaFKBP39 + pB42ADUSP (9), pGildaChd64 + pB42ADUSP (10), PGildaFKBP39 + pB42ADMet (11), pGild-aChd64 + pB42ADMet (12), pGildaEcR (13), pGildaUSP (14), pGildaMet (15), pB42ADEcR (16), pB42ADUSP (17), and pB42ADMet (18). The expression of the two-hybrid target gene, β-galactosidase, was monitored by filter assays using X-gal as the enzyme substrate. Expression of β-galactosidase turned yeast colonies blue in the presence of X-gal, indicating physical interactions between the two proteins tested.

Journal: The Journal of biological chemistry

Article Title: Identification and Characterization of a Juvenile Hormone Response Element and Its Binding Proteins *

doi: 10.1074/jbc.M704595200

Figure Lengend Snippet: The two-hybrid vectors pGilda and pB42AD contained the LexA DNA-binding domain and the B42 activation domain, respectively. Yeast EGY48 cells were transformed with either one or two plasmids as follows. A, pGildaFKBP39 (1), pB42ADChd64 (2), pGildaFKBP39 + pB42ADChd64 (3), pGildaChd64 (4), pB42ADFKBP39 (5), and pGildaChd64 + pB42ADFKBP39 (6). B, pGildaEcR + pB42ADFKBP39 (1), pGildaEcR + pB42ADChd64 (2), pGildaUSP + pB42ADFKBP39 (3), pGildaUSP + pB42ADChd64 (4), pGildaMet + pB42ADFKBP39 (5), pGildaMet + pB42ADChd64 (6), pGildaFKBP39 + pB42ADEcR (7), pGildaChd64 + pB42ADEcR (8), pGildaFKBP39 + pB42ADUSP (9), pGildaChd64 + pB42ADUSP (10), PGildaFKBP39 + pB42ADMet (11), pGild-aChd64 + pB42ADMet (12), pGildaEcR (13), pGildaUSP (14), pGildaMet (15), pB42ADEcR (16), pB42ADUSP (17), and pB42ADMet (18). The expression of the two-hybrid target gene, β-galactosidase, was monitored by filter assays using X-gal as the enzyme substrate. Expression of β-galactosidase turned yeast colonies blue in the presence of X-gal, indicating physical interactions between the two proteins tested.

Article Snippet: For the yeast two-hybrid interaction assay, we used the Clontech Yeast Matchmaker LexA system (BD Biosciences Clontech) with the LexA DNA-binding domain plasmid, pGilda, and the B42 activation domain plasmid, pB42AD, and lacZ reporter plasmid, p8op-lacZ, which carries the lacZ gene under the control of eight LexA operators and the minimal TATA region from the Gal1 promoter.

Techniques: Binding Assay, Activation Assay, Transformation Assay, Expressing